NGS (Next-Generation-Sequencing)

Available Library preparation for RNA Sequencing

  • Total RNA-sequencing
  •  mRNA-Sequencing
  • Low Input Total RNA-Sequencing

Please fill the form and send it to luxgen@lih.lu to receive a quote.

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Kit name

 

Company

 

Processing

 

Total RNA Quantity

 

Quality

 

Stranded

TruSeq Stranded Total RNA Library Prep Gold

 

Illumina

 

rRNA and mt-rRNA depletion available for a broad range of species

 

100 ng to 1µg

 

High, lower quality require optimization

 

YES

TruSeq® Stranded mRNA Library Prep

 

Illumina

 

mRNA selection For species with polyA tails

 

0.1 – 1 ug total RNA or 10 - 100 ng previously isolated mRNA (from species with polyA tails)

 

High quality

  YES

Trio RNA-Seq Library Preparation Kit

 

Nugen

 

Depletion to any transcript from any organism

 

500 pg - 50 ng

 

High quality and moderately degraded

  NO

QuantSeq 3´mRNA seq Fwd Library Prep Kit

 

Lexogen

 

Oligo d(T) priming UMI optional Mt-rRNA depletion optional

 

2 ng – 2 µg 500 ng recommended

 

Free of contaminants High quality and moderately degraded

  YES

NEBNext Multiplex Small RNA Library Prep

 

New England Biolabs

 

Gel Size selection

 

125 ng – 1 µg total RNA

 

High Quality RNA

  YES

 

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Library preparation and data analysis are done at LIH. Library Sequencing is done at LNS.